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Counting Chambers
Fuchs-Rosenthal
The Fuchs-Rosenthal Counter is one piece construction ensuring durability and accuracy, featuring a Fuchs-Rosenthal ruling on a single plateau. TheFuchs-Rosenthal Counting Chamber is used for Cerebro-Spinal Fluid cell counts. (Cell-Depth 0.2mm)
Photograph of 3720
| Catalog # |
Description |
| 3720 |
Counting chamber with 2 cover slips |
| 5000 |
Replacement Cover Glass |
|
Directions for Use
Fuchs-Rosenthal Chamber Catalog Number: 3720
Usage: Cell Counts in Cerebro-Spinal Fluid
Cell Depth: 0.2mm
Volume: 0.2 Microliters
Ruling Pattern: Fuchs-Rosenthal (Dark-Line)
Diluting fluid for counting cells in Cerebro-Spinal Fluid
Crystal or Methyl Violet...................................0.2 gm
Glacial Acetic Acid............................................10 cc
Distilled Water...................................................90 cc
Collect about five CC. of spinal fluid in a sterile test tube containing a trace of Potassium Oxalate to prevent coagulation. The specimen should contain no visible trace of blood. Fill the capillary of a 1:10 pipette to the graduation with the diluting fluid and then draw the spinal fluid to the graduation above the bulb. Shake the pipette and charge the chamber as with blood corpuscle counting technique. Allow several minutes for settling. The chambers are ruled with the Fuchs-Rosenthal pattern. This consists of 16 one square millimeter areas orientated by triple lines, and each such area sub-divided into 16 squares. It is generally recommended to count 16 one square millimeter areas preferably 8 in each chamber.
Neubauer Ruling
Calculating Formula if 16 one square millimeter areas were counted:
Number of cells counted X 10/9 = Cells per CMM
3.2
For convenient calculation divide the cells counted by 3 instead of 3.2 and disreguard the 10/9 dilution. The two fractions will compensate each other so that the remaining error will be negligible.
If the entire ruled area of both cells was counted, divide the results of the above formula by two to obtain the cells per CMM.
To clean the counting chamber: After completing the count, remove the cover glass and clean the counting chamber with water or a mild cleaning solution (10% solution of bleach). Dry the counting chamber with a soft cloth or wipe, or rinse with acetone.
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